InSplorer 内窥镜


InSplorer 探针内窥镜支持双光子/三光子自荧光成像,二次/三次谐波成像,以及反斯托克斯相干拉曼成像。

CARS/SHG – Humain Colon Lipid(Red) and Collagen(Green)
CARS – Human Colon Lipid
Mouse spinal cord: Myelin (magenta – CARS), spinal axon (blue – 2photon)
Human skin: stratum corneum (autofluorescence)
Mouse brain labelled with GCamp (2photon)
Collagen fibers: rat tail tendon – SHG
Epidermal layer of unstained mouse skin. Collagen fibrils structure (SHG – green) ; Cellular structure (3PEF – red, THG – blue)
GFP labeled mouse brain (3PEF – λexc = 1300 nm)
(a) Unstained mouse retina (3PEF – λexc = 1300 nm) radial section; (b) Ganglion cell layer (GCL) transverse section; (c) Inner plexiform layer (IPL) transverse section

InSplorer 集成系统内部包括激光光源,光电设备和电脑,外部通过FemtoGuide光纤连接至仅4厘米长,直径2.2毫米的探针。InSplorer同时配有友好的用户操作软件和交互界面。

InSplorer 系统可根据客户需求,同时支持多类通用多光子激光器,使用 FemtoGuide 光纤连接至探针。


  • Pulsed femtosecond and picosecond lasers
  • Wavelength range: 750 nm to 1550 nm
  • Integration of your existing light source using our FemtoGuide Fiber
  • Frame rate: up to 10 Hz
  • Field of view: up to 600 µm
  • PSF: 1 µm
  • Collection NA: up to 0.7
  • Working distance: up to 600 µm
  • Imaging modalities (Fluorescence and label free): 2-Photon, 3-photon, SHG, THG, CARS
  • Tip diameter: 1.3 mm or 2.2 mm (distal)
  • Distal end length: <40 mm
  • Flexible part length: 50-400 cm
  • Weight (distal end): < 2gr


D. Septier, V. Mytskaniuk, R. Habert, D. Labat, K. Baudelle, A. Cassez, G. Brévalle-Wasilewski, M. Conforti, G. Bouwmans, H. Rigneault, and A. Kudlinski, “Label-free highly multimodal nonlinear endoscope,” Optics Express 30, 25020-25033 (2022).

A. Kudlinski, A. Cassez, O. Vanvincq, D. Septier, A. Pastre, R. Habert, K. Baudelle, M. Douay, V. Mytskaniuk, V. Tsvirkun, H. Rigneault, and G. Bouwmans, “Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy,” Optics Express 28, 15062-15070 (2020).

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Science & Applications 7, 10 (2018).

A. Lombardini, E. R. Andresen, A. Kudlinski, I. Rimke, and H. Rigneault, “Origin and suppression of parasitic signals in Kagomé lattice hollow core fibers used for SRS microscopy and endoscopy,” Optics Letters 42, 1824-1827 (2017).