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M-FIP

Multiphoton Flexible Imaging Probe: M-FIP
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Flexible Probe for Multiphoton Multimodal Imaging

The M-FIP multiphoton endoscope enables multimodal nonlinear imaging such as 2-photon and 3-photon fluorescence but also harmonic generation (SHG, THG) and coherent Raman imaging (CARS).

The M-FIP probe is connected to a standalone cabinet that houses the laser, opto-electronics and computer. The M-FIP system is powered by a dedicated, user-friendly software.

Available laser sources:
  • Pulsed femtosecond or picosecond
  • Wavelength range: 680-1300 nm
  • Tunable repetition rate
  • Integration of your existing light source
Imaging performances:
  • Frame rate: up to 10 Hz
  • Field of view: up to 400 µm
  • Lateral resolution: 0.8 µm
  • Collection NA: 0.5
  • Working distance: 10 µm to 600 µm (fixed), 10 µm to 300 µm (tunable)
  • Imaging modalities (Fluorescence and label free): 2Photon, SHG, THG, CARS
Microendoscope specifications:
  • Tip diameter: 2.2 mm
  • Length (rigid part): 38 mm
  • Flexible part length: 50-150 cm
  • Axial resolution: 6 µm
  • Weight: < 2gr
  • Working distance: 10 µm to 600 µm (fixed), 10 µm to 300 µm (tunable)
  • Imaging modalities (Fluorescence and label free): 2Photon, SHG, THG, CARS
M-FIP Endoscope Lightcore Technologies
M-FIP Endoscope Lightcore Technologies

Examples of multimodal nonlinear endoscopy in biological tissues

CARS/SHG (human colon)
Human colon tissue: fat (red - CARS), collagen (green - SHG)
CARS (human colon)
Fat tissue (human colon) - CARS
CARS/TPEF (mouse spinal cord)
Mouse spinal cord: Myelin (magenta - CARS), spinal axon (blue – 2photon)
Autofluorescence (human skin)
Human skin: stratum corneum (autofluorescence)
TPEF (mouse brain)
Mouse brain labelled with GCamp (2photon)
SHG (rat tail tendon)
Collagen fibers: rat tail tendon – SHG

Related publications

  • A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, "High-resolution multimodal flexible coherent Raman endoscope," Light: Science & Applications 7, 10 (2018). doi.org/10.1038/s41377-018-0003-3
  • A. Kudlinski, A. Cassez, O. Vanvincq, D. Septier, A. Pastre, R. Habert, K. Baudelle, M. Douay, V. Mytskaniuk, V. Tsvirkun, H. Rigneault, and G. Bouwmans, "Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy," Optics Express 28, 15062-15070 (2020). doi.org/10.1364/OE.389084