Shot-noise Limited Dual-Color Coherent Raman Scattering Microscopy: DC-CRS
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Shot-noise Limited Dual-Color Coherent Raman Scattering Microscopy

The DC-CRS microscope enables multimodal nonlinear imaging such as 2-photon, second harmonic generation (SHG), coherent Raman imaging (Stimulated Raman Scattering SRS and Coherent Anti-Stokes Raman Scattering CARS).

The DC-CRS includes a versatile laser source system enabling to target any molecular vibrational frequency in the [750 cm-1, 5700 cm-1] range and to perform ratio-metric measurement between two vibrational frequencies at the same time and at the same location. The system is powered with an intuitive and user-friendly software enabling simultaneous SHG and SRS/CARS imaging.

DC-CRS allows ultra-fast chemical imaging suitable to perform:

  • - Virtual histology of fixed or fresh tissues samples (Stimulated Raman Histology SRH)
  • - Chemical mapping of pharmaceutical tablets
  • - Active molecule/Drug penetration in skin and tissue
  • - Chemical cell imaging
  • - Deuterium, alkyne, nitrile chemical group imaging
  • - Metabolic imaging

Type of source: Automated picosecond (narrow-band) tunable light source
  • Shot-noise limited performance
  • Wavelenght 1: 1024±2 nm (fixed)
  • Wavelenght 2: 1034±2 nm (fixed)
  • Wavelenght 3: 650 – 950 nm (tunable)
  • Power 1: > 100 mW
  • Power 2: > 100 mW
  • Power 3: > 150 mW at 800 nm
  • Repetition rate: 75.5 MHz
  • Pulse width: 1 ps
  • Coherent Raman tuning range: 750 – 5700 cm-1
  • Spectral bandwidth: 10 cm-1
Microscope: laser scanning microscope
  • Field of view : up to 300µm x 300µm
  • Excitation objective: 40X NA1.1
  • Collection objective: 40X NA1.1
  • Lateral resolution: ≤ 0.35 µm (FWHM at 800 nm)
  • Axial resolution: ≤ 1 µm (FWHM at 800 nm)
  • Pixel dwell time: ≥ 2 µs; up to 2 frames/s (image size: 500x500)
  • Axial scanning range: 250 µm
System dimensions:
  • Size: 1.4 m x 1.2 m x 0.8 m (table top design)
  • Size: 1 m x 1.2 m x 1.3 m (compact design)
Table top design
Compact design

Examples of DC-CRS and multimodal images

Stimulated Raman histology
Stimulated Raman histology (a), comparaison with standard histology (b) and overlay (c). Scale bar 100 µm
SRS (pharmaceutical tablets) SRS (pharmaceutical tablets)
Polymorphs (A-F1; A-F2) and excipient imaging in pharmaceutical tablets (SRS), 1 mm x 1 mm
CARS/autofluorescence (human skin) CARS/autofluorescence (human skin)
Active molecule penetration in human skin (red - CARS, green – autofluorescence) – 60 µm x 60 µm
CARS/2photon (mouse spinal cord)
Multimodal imaging in mouse spinal cord: magenta: myelin (CARS), blue: axons (CFP-2photon), yellow: macrophages (YFP-2photon), green: immune cells (GFP-2photon) (1 mm x 1 mm)
SHG/2photon (cell migration)
Cell migration (red: 2photon autofluorescence) on collagen scaffold (green: SHG)