InSplorer Endoscope

Flexible Probe for Multiphoton Multimodal Imaging

InSplorer endoscope enables multimodal nonlinear imaging such as 2-photon and 3-photon fluorescence but also harmonic generation (SHG, THG) and coherent Raman imaging (CARS).

CARS/SHG – Humain Colon Lipid(Red) and Collagen(Green)
CARS – Human Colon Lipid
Mouse spinal cord: Myelin (magenta – CARS), spinal axon (blue – 2photon)
Human skin: stratum corneum (autofluorescence)
Mouse brain labelled with GCamp (2photon)
Collagen fibers: rat tail tendon – SHG
Epidermal layer of unstained mouse skin. Collagen fibrils structure (SHG – green) ; Cellular structure (3PEF – red, THG – blue)
GFP labeled mouse brain (3PEF – λexc = 1300 nm)
(a) Unstained mouse retina (3PEF – λexc = 1300 nm) radial section; (b) Ganglion cell layer (GCL) transverse section; (c) Inner plexiform layer (IPL) transverse section

InSplorer probe is connected to a standalone cabinet that houses the laser, opto-electronics and computer. The InSplorer system is powered by a dedicated, user-friendly software.

InSplorer multiphoton endoscope can also use your favorite table-top multiphoton laser using the Lightcore FemtoGuide Fiber

Main Specifications

Available laser sources:
  • Pulsed femtosecond and picoseconds lasers
  • Wavelength range: 750nm to 1550 nm
  • Integration of your existing light source using our multiphoton delivery fiber (M-DF)
Imaging performances:
  • Frame rate: up to 10 Hz
  • Field of view: up to 400 µm
  • Lateral resolution: 1 µm
  • Collection NA: 0.5
  • Working distance: 10 µm to 600 µm (fixed), 10 µm to 300 µm (tunable)
  • Imaging modalities (Fluorescence and label free): 2-Photon, SHG, THG, CARS
Microendoscope specifications:
  • Tip diameter: 1.3 mm or 2 mm (distal)
  • Distal end length: <40 mm
  • Flexible part length: 50-400 cm
  • Lateral resolution: 1 µm
  • Axial resolution: 6 µm
  • Weight (distal end): < 2gr
  • Working distance: 10 µm to 600 µm (fixed), 10 µm to 300 µm (tunable)


D. Septier, V. Mytskaniuk, R. Habert, D. Labat, K. Baudelle, A. Cassez, G. Brévalle-Wasilewski, M. Conforti, G. Bouwmans, H. Rigneault, and A. Kudlinski, “Label-free highly multimodal nonlinear endoscope,” Optics Express 30, 25020-25033 (2022).

A. Kudlinski, A. Cassez, O. Vanvincq, D. Septier, A. Pastre, R. Habert, K. Baudelle, M. Douay, V. Mytskaniuk, V. Tsvirkun, H. Rigneault, and G. Bouwmans, “Double clad tubular anti-resonant hollow core fiber for nonlinear microendoscopy,” Optics Express 28, 15062-15070 (2020).

A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, “High-resolution multimodal flexible coherent Raman endoscope,” Light: Science & Applications 7, 10 (2018).

A. Lombardini, E. R. Andresen, A. Kudlinski, I. Rimke, and H. Rigneault, “Origin and suppression of parasitic signals in Kagomé lattice hollow core fibers used for SRS microscopy and endoscopy,” Optics Letters 42, 1824-1827 (2017).