BondXplorer Microscope

Shot-Noise Limited Dual-Color stimulated Raman Scattering Microscopy

BondXplorer microscope enables multimodal nonlinear imaging such as 2-photon, second harmonic generation (SHG), coherent Raman imaging (Stimulated Raman Scattering SRS and Coherent Anti-Stokes Raman Scattering CARS).

BondXplorer includes a versatile laser source system enabling to target two molecular vibrational frequencies in the [350 cm-1, 3800 cm-1] range and to perform ratio-metric measurement between these two vibrational frequencies at the same time and at the same location. The system is powered with an intuitive and user-friendly software enabling simultaneous 2-photon, SHG and SRS/CARS imaging.

BondXplorer allows ultra-fast chemical imaging suitable to perform:

– Virtual histology of fixed or fresh tissues samples (Stimulated Raman Histology SRH)

– Chemical mapping of pharmaceutical tablets

– Active molecule/Drug penetration in skin and tissue

– Chemical cell imaging

– Deuterium, alkyne, nitrile chemical group imaging

– Metabolic imaging


Lightcore Technologies BondXplorer microscope is powered by the deltaEmerald laser source from A.P.E


Main Specifications

TYPE OF SOURCE: AUTOMATED PICOSECOND (NARROW-BAND) TUNABLE LIGHT SOURCE
  • Shot-noise limited performance
  • Wavelenght 1: 1034±2 nm (fixed)
  • Wavelenght 2: 1024±2 nm (fixed)
  • Wavelength Pump (OPO Signal ) : 740 ~ 990 nm (tunable)
  • ∆v Pump – Stokes range : 350 cm-1 ~ 3800 cm-1
  • Power Stokes 1: > 300 mW (unmodulated), >150 mW modulated (20 MHz)
  • Power Stokes 2: > 300 mW (unmodulated), >150 mW modulated (13 MHz)
  • Power Pump (OPO Signal): > 400 mW at 800 nm
  • Repetition rate: 80 MHz
  • Pulse width: 1 ps
  • Spectral bandwith : 10 cm-1 ~ 15 cm-1
MICROSCOPE: LASER SCANNING MICROSCOPE
  • Field of view : up to 350 µm x 350 µm (mm2 using mosaicing)
  • Excitation objective: 25X NA1.1
  • •Collection condenser: abbe 0.90
  • Lateral resolution: ≤ 0.35 µm (FWHM at 800 nm)
  • Axial resolution: ≤ 1 µm (FWHM at 800 nm)
  • Pixel dwell time: ≥ 2 µs; up to 2 frames/s (image size: 500×500)
  • Axial scanning range: 250 µm
SYSTEM DIMENSIONS (L x W x H)
  • 1.5 m x 1.1 m x 0.7 m (table top design)
  • 0.6 m x 1..1 m x 1.3 m (compact design)

TABLE TOP DESIGN


Compact design


Check Out Its Diverse Applications


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