Shot-Noise Limited Dual-Color stimulated Raman Scattering Microscopy
BondXplorer microscope enables multimodal nonlinear imaging such as 2-photon, second harmonic generation (SHG), coherent Raman imaging (Stimulated Raman Scattering SRS and Coherent Anti-Stokes Raman Scattering CARS).
BondXplorer includes a versatile laser source system enabling to target two molecular vibrational frequencies in the [350 cm-1, 3800 cm-1] range and to perform ratio-metric measurement between these two vibrational frequencies at the same time and at the same location. The system is powered with an intuitive and user-friendly software enabling simultaneous 2-photon, SHG and SRS/CARS imaging.
BondXplorer allows ultra-fast chemical imaging suitable to perform:
– Virtual histology of fixed or fresh tissues samples (Stimulated Raman Histology SRH)
– Chemical mapping of pharmaceutical tablets
– Active molecule/Drug penetration in skin and tissue
– Chemical cell imaging
– Deuterium, alkyne, nitrile chemical group imaging
– Metabolic imaging
Lightcore Technologies BondXplorer microscope is powered by the deltaEmerald laser source from A.P.E
Main Specifications
TYPE OF SOURCE: AUTOMATED PICOSECOND (NARROW-BAND) TUNABLE LIGHT SOURCE
- Shot-noise limited performance
- Wavelenght 1: 1034±2 nm (fixed)
- Wavelenght 2: 1024±2 nm (fixed)
- Wavelength Pump (OPO Signal ) : 740 ~ 990 nm (tunable)
- ∆v Pump – Stokes range : 350 cm-1 ~ 3800 cm-1
- Power Stokes 1: > 300 mW (unmodulated), >150 mW modulated (20 MHz)
- Power Stokes 2: > 300 mW (unmodulated), >150 mW modulated (13 MHz)
- Power Pump (OPO Signal): > 400 mW at 800 nm
- Repetition rate: 80 MHz
- Pulse width: 1 ps
- Spectral bandwith : 10 cm-1 ~ 15 cm-1
ADDITIONAL FEMTOSECOND OUTPUT
- Wavelength: 1030 nm ± 3 nm
- Power: 300 mW
- Pulse width: < 200 fs
- Beam divergence: < 1.5 mrad
MICROSCOPE: LASER SCANNING MICROSCOPE
- Field of view : up to 350 µm x 350 µm (mm2 using mosaicing)
- Excitation objective: 25X NA1.1
- •Collection condenser: abbe 0.90
- Lateral resolution: ≤ 0.35 µm (FWHM at 800 nm)
- Axial resolution: ≤ 1 µm (FWHM at 800 nm)
- Pixel dwell time: ≥ 2 µs; up to 2 frames/s (image size: 500×500)
- Axial scanning range: 250 µm
SYSTEM DIMENSIONS (L x W x H)
- 1.5 m x 1.1 m x 0.7 m (table top design)
- 0.6 m x 1..1 m x 1.3 m (compact design)
TABLE TOP DESIGN
Compact design
