Chemical Imaging (Pharmaceutics/Cosmetics)

Mapping Chemical Compounds Distribution in Pharmaceutical/Cosmetics Formulations, and Monitoring Active Molecular Penetration in Skin

You are a chemical scientist and you want to image in a label-free manner chemical compounds in complex pharmaceutical tablets or cosmetics formulation. Lightcore Technologies BondXplorer microscope provides a versatile and unable platform to image chemical compounds through their vibrational signature [350cm-1, 3800cm-1] and monitor deuterated active molecular penetration in skin ex vivo and in vivo.

BondXplorer: Image of pharmaceutical tablet containing three excipients (PEG, Manitol, Starch) and two polymorph active molecules (A-F1 and A-F2).

You need the versatility of a flexible probe to image in restrained or inaccessible environment. Lightcore Technologies InSplorer flexible endoscope with its 2 mm distal diameter performs label-free CARS microscopy, second and third harmonic generation, and 2photon fluorescence or auto-fluorescence of complex samples such as skin.

BondXplorer microscope: Active cosmetic molecule (red) as seen by CARS imaged in reconstructed skin (green) as seen by 2-photon auto-fluorescence

BondXplorer microscope: Deuterated glycerol concentration in in vivo human skin with depth as measured by CARS 1h10m after application

BondXplorer microscope: Active formulation thickness (in micron) after being spread over PMMA skin imprint. Field of view of 1.4 mm x 1.1 mm

InSplorer endoscope imaging of stratum corneum

Related Publications:

  • B. Sarri, R. Canonge, X. Audier, V. Lavastre, G. Pénarier, J. Alie, and H. Rigneault, “Discriminating polymorph distributions in pharmaceutical tablets using stimulated Raman scattering microscopy,” Journal of Raman Spectroscopy 50, 1896-1904 (2019).
  • B. Sarri, X. Chen, R. Canonge, S. Grégoire, F. Formanek, J-B Galey, AnnePotter, T. Bornschlögl, and H. Rigneault, ‘In vivo quantitative molecular absorption of glycerol in human skin using coherent anti-Stokes Raman scattering (CARS) and two-photon auto-fluorescence’, J Controlled Release 308, 190-196 (2019)
  • X. Chen, P. Gasecka, F. Formanek, J. B. Galey, and H. Rigneault, ‘In vivo single human sweat gland activity monitoring using coherent anti-Stokes Raman scattering and two-photon excited auto-fluorescence microscopy’, British Journal of Dermatology 174, 803–812, (2016)
    Highlighted in BJD: S. Osseiran, C. L. Evans, Sweating the small stuff, British J. Dermat. 174, 709 (2016)
  • X. Chen, S. Grégoire, F. Formanek, J.-B. Galey, H. Rigneault, ‘Quantitative 3D molecular cutaneous absorption in human skin using label free nonlinear microscopy’, Journal of Controlled Release 200, 78-86 (2015)