Imaging lipid order in living tissues:
When associated to polarization-resolved measurement schemes,
Lightcore Technologies DC-CRS microscope
provides label-free chemical bond orientation in complex system such as mouse myelin sheath.
resolved in polarization reveals the orientation of C-H bonds in mouse Myelin sheath.
Field of view 150 µm x 150 µm.
P. Gasecka, A. Jaouen, F.-Z. Bioud, H. B. de Aguiar, J. Duboisset, P. Ferrand, H. Rigneault, N. K. Balla,
F. Debarbieux, and S. Brasselet, "Lipid Order Degradation in Autoimmune Demyelination Probed by Polarized
Coherent Raman Microscopy," Biophysical Journal 113, 1520-1530 (2017).
C. Cleff, A. Gasecka, P. Ferrand, H. Rigneault, S. Brasselet, and J. Duboisset, "Direct imaging of molecular
symmetry by coherent anti-stokes Raman scattering," Nat Commun 7, 11562 (2016).