Instantaneous Histology:
You are a clinical practitioner, a pathologist or a scientist that wants to have access to the histological
information from a fresh biopsiy tissue. Lightcore Technologies
DC-CRS microscope provides
immediate label-free histological imaging over mm2 field of view in few minutes only.
No sample preparation, no staining, instantaneous label free histology is possible with our DC-CRS microscope.
(a) Stimulated Raman histology (SRH) as provided by the DC-CRS microscope,
(b) comparison of the same area using classical H&E staining,
(c) SRH image overlaid on the H&E image for comparison.
Related publications:
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B. Sarri, F. Poizat, S. Heuke, J. Wojak, F. Franchi, F. Caillol, M. Giovannini, and H. Rigneault,
"Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining,"
Biomed. Opt. Express 10, 5378-5384 (2019).
doi.org/10.1364/BOE.10.005378
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B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat,
M. Giovannini, and H. Rigneault, "Fast stimulated Raman and second harmonic generation imaging for
intraoperative gastro-intestinal cancer detection," Scientific Reports 9, 10052 (2019).
doi.org/10.1038/s41598-019-46489-x
Tissue architectures in vivo:
You are a life science scientist that wants to image tissue architecture in vivo on animal model (mouse,…).
Lightcore Technologies
M-FIP flexible endoscope,
2mm distal diameter, provides up to 8 frames/s imaging of a variety of contrasts such as 2photon fluorescence
and auto-fluorescence, harmonic generations (SHG and TGH) to locate collagen and interfaces, but also coherent
Raman (CARS) to see lipids label free.
M-FIP flexible endoscope imaging:
(a) Fat tissues (human colon), (b) collagen (rat tail tendon),
(c) zoom in (b) showing the sub-micron resolution, (d) and (e) collagen (green)
and lipid (red) in excised biopsies samples from human colon.
Related publications:
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A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly,
F. Louradour, A. Kudlinski, and H. Rigneault, "High-resolution multimodal flexible coherent Raman
endoscope," Light: Science & Applications 7, 10 (2018).
doi.org/10.1038/s41377-018-0003-3