You are a clinical practitioner, a pathologist or a scientist that wants to have access to the histological
information from a fresh biopsiy tissue. Lightcore Technologies
DC-CRS microscope provides
immediate label-free histological imaging over mm2 field of view in few minutes only.
No sample preparation, no staining, instantaneous label free histology is possible with our DC-CRS microscope.
(a) Stimulated Raman histology (SRH) as provided by the DC-CRS microscope,
(b) comparison of the same area using classical H&E staining,
(c) SRH image overlaid on the H&E image for comparison.
B. Sarri, F. Poizat, S. Heuke, J. Wojak, F. Franchi, F. Caillol, M. Giovannini, and H. Rigneault,
"Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining,"
Biomed. Opt. Express 10, 5378-5384 (2019).
B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat,
M. Giovannini, and H. Rigneault, "Fast stimulated Raman and second harmonic generation imaging for
intraoperative gastro-intestinal cancer detection," Scientific Reports 9, 10052 (2019).
Tissue architectures in vivo:
You are a life science scientist that wants to image tissue architecture in vivo on animal model (mouse,…).
M-FIP flexible endoscope,
2mm distal diameter, provides up to 8 frames/s imaging of a variety of contrasts such as 2photon fluorescence
and auto-fluorescence, harmonic generations (SHG and TGH) to locate collagen and interfaces, but also coherent
Raman (CARS) to see lipids label free.
M-FIP flexible endoscope imaging:
(a) Fat tissues (human colon), (b) collagen (rat tail tendon),
(c) zoom in (b) showing the sub-micron resolution, (d) and (e) collagen (green)
and lipid (red) in excised biopsies samples from human colon.
A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly,
F. Louradour, A. Kudlinski, and H. Rigneault, "High-resolution multimodal flexible coherent Raman
endoscope," Light: Science & Applications 7, 10 (2018).