Medical / Biomedical Applications

Instantaneous Histology:

You are a clinical practitioner, a pathologist or a scientist that wants to have access to the histological information from a fresh biopsiy tissue. Lightcore Technologies DC-CRS microscope provides immediate label-free histological imaging over mm2 field of view in few minutes only.

No sample preparation, no staining, instantaneous label free histology is possible with our DC-CRS microscope.

Stimulated Raman histology
(a) Stimulated Raman histology (SRH) as provided by the DC-CRS microscope, (b) comparison of the same area using classical H&E staining, (c) SRH image overlaid on the H&E image for comparison.

Related publications:

  • B. Sarri, F. Poizat, S. Heuke, J. Wojak, F. Franchi, F. Caillol, M. Giovannini, and H. Rigneault, "Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining," Biomed. Opt. Express 10, 5378-5384 (2019). doi.org/10.1364/BOE.10.005378
  • B. Sarri, R. Canonge, X. Audier, E. Simon, J. Wojak, F. Caillol, C. Cador, D. Marguet, F. Poizat, M. Giovannini, and H. Rigneault, "Fast stimulated Raman and second harmonic generation imaging for intraoperative gastro-intestinal cancer detection," Scientific Reports 9, 10052 (2019). doi.org/10.1038/s41598-019-46489-x

Tissue architectures in vivo:

You are a life science scientist that wants to image tissue architecture in vivo on animal model (mouse,…). Lightcore Technologies M-FIP flexible endoscope, 2mm distal diameter, provides up to 8 frames/s imaging of a variety of contrasts such as 2photon fluorescence and auto-fluorescence, harmonic generations (SHG and TGH) to locate collagen and interfaces, but also coherent Raman (CARS) to see lipids label free.

M-FIP CARS and SHG imaging
M-FIP flexible endoscope imaging: (a) Fat tissues (human colon), (b) collagen (rat tail tendon), (c) zoom in (b) showing the sub-micron resolution, (d) and (e) collagen (green) and lipid (red) in excised biopsies samples from human colon.

Related publications:

  • A. Lombardini, V. Mytskaniuk, S. Sivankutty, E. R. Andresen, X. Chen, J. Wenger, M. Fabert, N. Joly, F. Louradour, A. Kudlinski, and H. Rigneault, "High-resolution multimodal flexible coherent Raman endoscope," Light: Science & Applications 7, 10 (2018). doi.org/10.1038/s41377-018-0003-3