Mapping active molecule distribution in real or artificial skin:
You are a chemical scientist and you want to image in a label-free manner chemical compounds in complex media
such as skin. Lightcore Technologies
DC-CRS microscope
provides label free imaging of skin in depth and monitors deuterated active molecule percutaneous penetration
ex vivo and in vivo.
You need the versatility of a flexible probe to image in restrained or inaccessible environment. Lightcore
Technologies M-FIP flexible endoscope
with its 2 mm distal diameter performs label-free CARS microscopy, second and third harmonic generation,
and 2photon fluorescence or auto-fluorescence of complex samples such as skin.
DC-CRS microscope:
Active cosmetic molecule (red) as seen by CARS imaged in reconstructed skin (green) as seen by 2photon
auto-fluorescence
DC-CRS microscope: Deuterated glycerol concentration in in vivo
human skin with depth as measured by CARS 1h10m after application
M-FIP flexible endoscope imaging of stratum corneum
Related publications:
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B. Sarri, X. Chen, R. Canonge, S. Grégoire, F. Formanek, J-B Galey, AnnePotter, T. Bornschlögl, and
H. Rigneault, ‘In vivo quantitative molecular absorption of glycerol in human skin using coherent anti-Stokes
Raman scattering (CARS) and two-photon auto-fluorescence’, J Controlled Release 308, 190-196 (2019)
doi.org/10.1016/j.jconrel.2019.07.018
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X. Chen, P. Gasecka, F. Formanek, J. B. Galey, and H. Rigneault, ‘In vivo single human sweat gland activity
monitoring using coherent anti-Stokes Raman scattering and two-photon excited auto-fluorescence microscopy’,
British Journal of Dermatology 174, 803–812, (2016)
doi.org/10.1111/bjd.14292
Highlighted in BJD: S. Osseiran, C. L. Evans, Sweating the small stuff, British J. Dermat. 174, 709 (2016)
doi.org/10.1111/bjd.14504
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X. Chen, S. Grégoire, F. Formanek, J.-B. Galey, H. Rigneault, ‘Quantitative 3D molecular cutaneous absorption
in human skin using label free nonlinear microscopy’, Journal of Controlled Release 200, 78-86 (2015)
doi.org/10.1016/j.jconrel.2014.12.033
Controlling how cosmetics active formulation map and spread on real or artificial skin:
DC-CRS Microscope:
Active formulation thickness (in micron) after being spread over
PMMA skin imprint. Field of view of 1.4 mm x 1.1 mm